Fluid Flow Induces Biofilm Formation in Staphylococcus epidermidis Polysaccharide Intracellular Adhesin-Positive Clinical Isolates
نویسندگان
چکیده
منابع مشابه
Detection of Intracellular Adhesion (ica) and Biofilm Formation Genes in Staphylococcus aureus Isolates from Clinical Samples
Background: The nosocomial infections that cause the establishment of biofilms on the embedded biomedical surfaces are the leading cause of sepsis and are often related to colonization of implants by Staphylococcus epidermidis. Materials and Methods: A total of 40 clinical S. aureus isolates were collected from Zabol, Iran. The ability of these strains to form biofilm was determined by microli...
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Biofilm production is an important step in the pathogenesis of Staphylococcus epidermidis polymer-associated infections and depends on the expression of the icaADBC operon leading to the synthesis of a polysaccharide intercellular adhesin. A chromosomally encoded reporter gene fusion between the ica promoter and the beta-galactosidase gene lacZ from Escherichia coli was constructed and used to ...
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Biofilm formation in Staphylococcus epidermidis is mediated by icaADBC-dependent and -independent pathways. Subinhibitory concentrations of erythromycin, azithromycin, and clarithromycin enhanced, in a dose-dependent manner, the level of biofilm formation by 20% (21/105 isolates) by macrolide-resistant ica-positive and -negative isolates tested in vitro. The presence of ica, however, apparently...
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Medical polymers, such as urinary catheters are widely used biomaterials. One of the main problem for using the urinary catheters is biofilm formation on their surface, when they are used in a long time in the body. Virulence and pathogenicity of Staphylococcus epidermidis is often enhanced when growing as a biofilm. Many techniques have been presented to reduce the biofilm formation by surface...
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b a ckground : in fact the biofilms are composed of bacterial cells living inmulticellular structures such as tissues and organs embedded within a self-produced matrix of extracellular polymeric substance (eps). ability to attach and biofilm formation are the most important virulence factors staphylococcus aureus isolates. the aims of this study were to detect intracellular adhesion (ica) locus...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2012
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01139-12